The complete ordering of the fifty letters, separated by syllable is:

I ro ha ni ho he to
chi ri nu ru wo
wa ka yo ta re so
tsu ne na ra mu
u wi no o ku ya ma
ke fu ko e te
a sa ki yu me mi shi
we hi mo se su, n

いろはにほへと
ちりぬるを
わかよたれそ
つねならむ
うゐのおくやま
けふこえて
あさきゆめみし
ゑひもせすん

We, and several other characters are no longer commonly used, and may be romanised differently.

To read it in modern sounds, it goes something like this:

iro wa nioedo
chirinuru o
waga yo tare zo
tsunenaran
ui no okuyama
kyou koete
asaki yume miji
yoi mo sezu. n.

The translation is:

Flowers, although fragrant, will be left behind.
Who in this world will remain immortal?
Today, we pass the high mountain
of illusions, there will be no more empty
dreaming and no more drunkenness. Un