The Beer-Lambert law (sometimes also known as the Beer-Lambert-Bouguer law or simply Beer's law) is used in spectroscopy. The law can be summed up by the mathematic relationship Aλ=ECL, where A is the absorbance at a given wavelength of light, E is the molar extinction coefficient, C is the concentration of the molar solution, and L is the length of the light path.

In words, the equation means that the concentration of a substance in moles is proportional to the absorption of a given wavelength of light by a solution of the substance.


From the BioTech Dictionary at http://biotech.icmb.utexas.edu/. For further information see the BioTech homenode.

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