In situ hybridization
is a molecular biology
technique that involves the use of a DNA
or RNA probe
to detect the presence of the complementary DNA
sequence in cloned bacterial
or cultured eukaryotic
This technique is also used for locating gene
s on chromosome
s. The process is:
- Prepare a microscope slide of the cells to be hybridized in the metaphase stage of mitosis.
- Treat the slide with a weak base; this denatures the cell's DNA.
- Pour a solution containing the radioactively labeled probe onto the slide.
- Expose slide to a photographic emulsion for a few days or weeks.
- Develop the emulsion.
From the BioTech Dictionary at http://biotech.icmb.utexas.edu/. For further information see the BioTech homenode.